Journal
CHEMICAL COMMUNICATIONS
Volume 56, Issue 38, Pages 5170-5173Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0cc01150a
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Funding
- Swiss commission for technology and innovation (CTI/KTI) [16437.1 PFENNM]
- European Commission, within the Horizon 2020 (PROLIFIC project, BBI-JU -grant) [790157]
- H2020 Societal Challenges Programme [790157] Funding Source: H2020 Societal Challenges Programme
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We report the first method of enzyme protection enabling the production of partially shielded enzymes capable of processing substrates as large as proteins. We show that partially shielded sortase retains its transpeptidase activity and can perform bioconjugation reactions on antibodies. Moreover, a partially shielded trypsin is shown to outperform its soluble counterpart in terms of proteolytic kinetics. Remarkably, partial enzyme shielding results in a drastic increase in temporal stability of the enzyme.
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