Journal
ISME JOURNAL
Volume 11, Issue 6, Pages 1305-1317Publisher
SPRINGERNATURE
DOI: 10.1038/ismej.2017.15
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Funding
- Alexander von Humboldt Foundation
- Swedish Research Council for Environment, Agricultural Sciences and Planning (FORMAS) [215-2010-779]
- University of Gothenburg
- Max Planck Society
- Daniel K Inouye Center for Microbial Oceanography: Research and Education (C-MORE)
- National Science Foundation [EF-0424599, OCE-1260164]
- Gordon and Betty Moore Foundation [3794]
- Simons Foundation (SCOPE) [329108]
- Swedish Research Council [821-2014-6375]
- Division Of Ocean Sciences
- Directorate For Geosciences [1260164] Funding Source: National Science Foundation
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Gradients of oxygen (O-2) and pH, as well as small-scale fluxes of carbon (C), nitrogen (N) and O-2 were investigated under different partial pressures of carbon dioxide (pCO(2)) in field-collected colonies of the marine dinitrogen (N-2)-fixing cyanobacterium Trichodesmium. Microsensor measurements indicated that cells within colonies experienced large fluctuations in O-2, pH and CO2 concentrations over a day-night cycle. O-2 concentrations varied with light intensity and time of day, yet colonies exposed to light were supersaturated with O-2 (up to similar to 200%) throughout the light period and anoxia was not detected. Alternating between light and dark conditions caused a variation in pH levels by on average 0.5 units (equivalent to 15 nmol l(-1) proton concentration). Single-cell analyses of C and N assimilation using secondary ion mass spectrometry (SIMS; large geometry SIMS and nanoscale SIMS) revealed high variability in metabolic activity of single cells and trichomes of Trichodesmium, and indicated transfer of C and N to colony-associated non-photosynthetic bacteria. Neither O-2 fluxes nor C fixation by Trichodesmium were significantly influenced by short-term incubations under different pCO(2) levels, whereas N-2 fixation increased with increasing pCO(2). The large range of metabolic rates observed at the single-cell level may reflect a response by colony-forming microbial populations to highly variable microenvironments.
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