Journal
IONICS
Volume 24, Issue 2, Pages 589-596Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11581-017-2199-2
Keywords
Electrostatic interaction; Electrochemical immunosensor; Gold nanorods; Charge-labeled
Funding
- Scientific Research Foundation of Shaanxi Provincial Key Laboratory [16JS101]
Ask authors/readers for more resources
The purpose of the research was to develop a proof-of-concept charge-labeled electrochemical immunosensor utilizing the electrostatic interaction of electrode surface. Gold nanorods, assembled with sulfonyl-terminated and carboxyl-terminated thiolated reagents, were applied as nanocarriers for negative charge and secondary antibodies due to their excellent biocompatibility and special interaction with mercapto groups. The sandwich-type immunosensor displayed excellent analytical performance including the broad linear concentration range of 1.0 fg mL(-)to 100 ng mL(-1) and a low detection limit of 1 fg mL(-1) for the detection of carcinoembryonic antigen. It was demonstrated that the electrostatic interaction contributed to the sensitivity of the immunosensor. Therefore, a proof-of-concept charge-labeled electrochemical immunosensor was proposed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available