Journal
INTERNATIONAL IMMUNOLOGY
Volume 32, Issue 3, Pages 213-219Publisher
OXFORD UNIV PRESS
DOI: 10.1093/intimm/dxz075
Keywords
diarrhea; food allergy; mast cells
Categories
Funding
- Japan Society for the Promotion of Science (KAKENHI) [16H06387, 18H05022]
- Ono Pharmaceutical Company Ltd.
- Grants-in-Aid for Scientific Research [18H05022] Funding Source: KAKEN
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Mast cells (MCs) play a critical role in oral allergen-induced anaphylaxis. However, the contribution of basophils to the anaphylaxis remains unclear. The inhibitory immunoreceptor Allergin-1 is highly expressed on MCs and basophils and inhibits Fc epsilon RI-mediated signaling in MCs. Here, we show that Allergin-1-deficient (Milr1(-/-)) mice developed more severe hypothermia, a higher mortality rate and a greater incidence of diarrhea than did wild-type (WT) mice in an oral ovalbumin (OVA)-induced food allergy model. MC-deficient Mas-TRECK mice, which had been reconstituted with either WT or Milr1(-/-) bone marrow-derived cultured MCs, did not develop hypothermia in this food allergy model. On the other hand, depletion of basophils by injection of anti-CD200R3 antibody rescued Milr1(-/-) mice from lethal hypothermia but not from diarrhea. In vitro analyses demonstrated that Allergin-1 inhibits IgE-dependent activation of both human and mouse basophils. Thus, Allergin-1 on basophils selectively suppresses oral allergen-induced anaphylaxis.
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