Journal
ANALYTICAL METHODS
Volume 12, Issue 18, Pages 2391-2397Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0ay00389a
Keywords
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Funding
- National Natural Science Foundation of China [21804050]
- Foundation from the Science and Technology Planning Project of Fujian Province of China [2016Y0064]
- Natural Science Foundation of Fujian Province of China [2019J05098, 2018J01432]
- Science and Technology Planning Project of Xiamen, China [3502Z20183031]
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A method for the aptamer-based determination of chloramphenicol (CAP) was developed by exploiting the peroxidase mimicking activity of hemin. The method includes two hemin-modified DNA probes termed P1 and P2. P1, which was modified at its 5 ' end with one hemin monomer, contains the CAP-binding sequence. The hybridization between P1 and P2 brings the two hemin monomers in close proximity, resulting in the formation of a hemin dimer with low peroxidase mimicking activity. The duplex structure was dehybridized in the presence of CAP. The formed hemin monomer featured a strong peroxidase mimicking activity and catalyzed the conversion of non-fluorescent tyramine into fluorescent dityramine by hydrogen peroxide. Fluorescence (with an excitation/emission maxima at 320 and 410 nm, respectively) increased linearly in the 0.1 ng mL(-1) to 10 ng mL(-1) CAP concentration range. The detection limit based on the 3 sigma/k criterion reached 0.07 ng mL(-1). The proposed assay was successfully employed for CAP detection in (spiked) honey samples with recoveries of 94.3-117.2%. Given its high sensitivity and good stability, this method shows potential in providing a platform for antibiotic detection.
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