Journal
JOURNAL OF BIOMIMETICS BIOMATERIALS AND BIOMEDICAL ENGINEERING
Volume 45, Issue -, Pages 49-56Publisher
TRANS TECH-SCITEC PUBLICATIONS LTD
DOI: 10.4028/www.scientific.net/JBBBE.45.49
Keywords
Bovine bone; extraction; hydroxyapatite; calcination; non-hazardous; fourier transform infrared; scanning electron microscopy; X-ray diffraction analysis; tissue engineering; scaffold
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Funding
- PDUPT grant from Indonesian Ministry of Research, Technology and Higher Education
- Tahir Professorship Program
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Bovine bone is a considerable source for the production of hydroxyapatite. The recent study reported a novel method to extract hydroxyapatite from bovine bone without producing hazardous residue. The bovine bones were cut and boiled in the opened chamber followed by boiling in pressurized tank. The bones were then soaked into 95% ethanol. Calcination was then conducted in 800 degrees C, 900 degrees C and 1,000 degrees C, for 2 hours. The result was then grinded and sieved. The powder then was characterized using Fourier transform infrared (FT-1R), Scanning electron microscopy (SEM) and X-ray diffraction analysis (XRD) to measure the purity of hydroxyapatite. It is concluded that the hydroxyapatite derived from this process showed 100% purity, resulting 35.34 +/- 0.39% w/w from the wet bone weight and 72.3% w/w from the dried weight. The present extraction method has been proven to yield high amount of pure hydroxyapatite as well as reducing the use of hazardous reagent.
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