Journal
LAB ON A CHIP
Volume 20, Issue 10, Pages 1845-1855Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0lc00302f
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Funding
- Natural Sciences and Engineering Research Council (NSERC)
- Abbott Laboratories
- Canadian Institutes for Health Research (CIHR)
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Finger-stick blood sampling is convenient for point of care diagnostics, but whole blood samples are problematic for many assays because of severe matrix effects associated with blood cells and cell debris. We introduce a new digital microfluidic (DMF) diagnostic platform with integrated porous membranes for blood-plasma separation from finger-stick blood volumes, capable of performing complex, multi-step, diagnostic assays. Importantly, the samples can be directly loaded onto the device by a finger dab for user-friendly operation. We characterize the platform by comparison to plasma generated via the gold standard centrifugation technique, and demonstrate a 21-step rubella virus (RV) IgG immunoassay yielding a detection limit of 1.9 IU mL(-1), below the diagnostic cut-off. We propose that this work represents a critical next step in DMF based portable diagnostic assays-allowing the analysis of whole blood samples without pre-processing.
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