4.7 Article

Homogeneous multiplexed digital detection of microRNA with ligation-rolling circle amplification

Journal

CHEMICAL COMMUNICATIONS
Volume 56, Issue 40, Pages 5409-5412

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/d0cc01530j

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Funding

  1. National Natural Science Foundation of China [21974078]

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A simple multiplexed digital microRNA detection strategy with fluorescence flow cytometry was proposed. By isothermal ligation-rolling circle amplification, multiplexed microRNAs could be simultaneously converted to a series of nanoflower balls (NFBs) and counted by flow cytometry directly.

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