Journal
CHEMICAL COMMUNICATIONS
Volume 56, Issue 40, Pages 5358-5361Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0cc01865a
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Funding
- Japan Science and Technology Agency under Adaptable and Seamless Technology Transfer Program through Target-driven RD (A-STEP)
- AMED [19am0401007]
- JSPS KAKENHI [JP18H03933, JP16H05925, JP17K14514]
- Asahi Glass Foundation
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A triplex-forming oligonucleotide (TFO) linear probe containing perylene derivatives was synthesized. The TFO linear probe formed a remarkably stable triplex with a target DNA duplex, resulting in the light-up of fluorescence emission. The sensitivity was extremely high even at pH 7. Detection of PCR-amplified target DNA was demonstrated.
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