Winogradskyella flava sp nov., isolated from the brown alga, Sargassum fulvellum

An aerobic, gliding and yellow-pigmented bacterium, designated strain SFD31(T), was isolated from brown alga collected from the South Sea, Republic of Korea. Cells were Gram-stain-negative, and catalase-and oxidase-positive. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain SFD31(T) forms an independent lineage within the genus Winogradskyella. Strain SFD31(T) was related distantly to Winogradskyella echinorum KMM 6211(T) (97.9 %, 16S rRNA gene sequence similarity), Winogradskyella litoriviva KMM 6491(T) (97.4 %), Winogradskyella pulchriflava EM106(T) (97.2 %) and Winogradskyella eckloniae EC29(T) (96.9 %). The major fatty acids of strain SFD31 T were iso-C-15:0, iso-C(15:1)G, summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c) and unknown 13.565. The only isoprenoid quinone of the isolate was menaquinone 6. The major polar lipids were phosphatidylethanolamine, four unidentified aminolipids and two unidentified lipids. The DNA G+C content of strain SFD31(T) was 36.0 mol%. Phenotypic characteristics distinguished strain SFD31(T) from the related species of the genus Winogradskyella. On the basis of the evidence presented in this study, a novel species, Winogradskyella flava sp. nov., is proposed for strain SFD31(T) (= KCTC 52348(T) = JCM 31798(T)).