Nocardioides phosphati sp nov., an actinomycete isolated from a phosphate mine

A Gram-staining-positive, non-spore-forming and non-motile strain, designated WYH11-7(T), was isolated from a phosphate mine in Yunnan Province, PR China. The taxonomic position of WYH11-7(T) was investigated by polyphasic approaches. Phylogenetic analyses based on 16S rRNA gene sequences indicated that WYH11-7(T) represents a member of the genus Nocardioides. WYH11-7(T) was closely related to Nocardioidesjensenii DSM 20641(T), Nocardioidesdubius DSM 19084(T) and Marmoricolaterrae DSM 27141T, and had pairwise 16S rRNA gene sequence similarities of 97.4, 97.2 and 97.0 %, respectively. DNA-DNA relatedness values between WYH11-7(T) and related type strains N. jensenii DSM 20641(T) and N. dubius DSM 19084(T) were found to be 17.6 +/- 4.9 and 14.6 +/- 3.1 %, respectively. The respiratory menaquinone of WYH11-7T was MK-8 (H4) while the major fatty acids were C-18:1 omega 9c, C-16:0, C-17:0, C-17:1 omega 8c, C18 : 1 10-methyl and summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unidentified phospholipids. Whole- cell hydrolysates contained mannose, ribose, glucose and galactose along with LL-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan. The DNA G+C content was 71.2 mol%. Phenotypic, phylogenetic and chemotaxonomic data indicated that strain WYH11-7(T) represents a novel species of the genus Nocardioides, for which the name Nocardioidesphosphatisp. nov. is proposed. The type strain is WYH11-7(T) (=CGMCC 4.7371(T)=DSM 104026(T)).