Paralkalibacillus indicireducens gen., nov., sp nov., an indigo-reducing obligate alkaliphile isolated from indigo fermentation liquor used for dyeing

Obligately alkaliphilic, indigo-reducing strains, designated Bps-1 T, Bps-2 and Bps-3, were isolated from an indigo fermentation liquor used for dyeing, which was produced from sukumo (composted Polygonum indigo leaves) obtained from a craft centre in Data City, Hokkaido, Japan, by using medium containing cellulase-treated sukumo. The 16S rRNA gene sequence phylogeny suggested that Bps-1 T has a distinctive position among the alkaliphilic species of the genus Bacillus, with its closest neighbours being Bacillus pseudofirmus DSM 8715(T), Bacillus lindianensis DSM 26864(T) and Bacillus alcalophilus DSM 485(T) (96.1, 95.8 and 95.5% 16S rRNA gene sequence similarities, respectively). The 16S rRNA sequence of strain Bps-1(T) was identical to those of strains Bps-2 and Bps-3. Cells of the novel isolate were Gram-stain-positive and were facultatively anaerobic straight rods that were motile by means of a pair of flagella (subpolar and centre sides). Spherical endospores were formed in the terminal position. Strain Bps-1(T) grew between 18 and 40 degrees C with optimum growth at 33 degrees C. The isolate grew in the pH range 8-11, with optimum growth at pH 9-10. The isoprenoid quinone detected was menaquinone-7 (MK-7), and the DNA G+C content was 40.3 %. The whole-cell fatty acid profile (> 10 %) mainly consisted of anteiso-C-15 : 0, iso-C-15 : 0 and C-16 : 0. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, the isolates represent a novel species of a novel genus, for which the name Paralkalibacillus indicireducens gen. nov., sp. nov. is proposed. The type strain of this species is Bps-1(T) (JCM 31808(T) = NCIMB 15080(T)), with strains Bps-2 and Bps-3 representing additional strains of the species.