Nonomuraea rhodomycinica sp nov., isolated from peat swamp forest soil

The taxonomic position of an actinomycete, strain NR4-ASC07(T), isolated from a soil sample collected from Sirindhorn peat swamp forest, Narathiwat Province, Thailand, was clarified using a polyphasic approach. On the basis of morphological and chemotaxonomic characteristics, it was classified among the members of the genus Nonomuraea. It produced tightly closed spiral spore chains on aerial mycelium as well as forming a pseudosporangium. Whole-cell hydrolysates contained meso-diaminopimelic acid, glucose, ribose, madurose and mannose. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, lyso-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, unknown ninhydrin-positive phosphoglycolipids and unknown glycolipid. Menaquiones were MK-9(H-4), MK-9(H-0), MK-9(H-2), MK-10(H-4) and MK-9(H-6). Predominant cellular fatty acids were iso-C-16 : 0, C-17 : 0 10-methyl, C-16 : 0, C-17 : 1 omega 8c, C-16 : 0 2-OH and iso-C-15 : 0. The phylogenetic tree reconstructed on the basis of 16S rRNA gene sequences showed that the strain fell within the clade containing Nonomuraea muscovyensis FMN03(T), Nonomuraea roseoviolacea subsp. roseoviolacea NBRC 14098(T) and Nonomuraea roseoviolacea subsp. carminata NBRC 15903(T). The DNA-DNA relatedness and phenotypic data supported that strain NR4-ASC07(T) was clearly distinguished from the closely related species and represents a novel species of the genus Nonomuraea for which the name Nonomuraea rhodomycinica sp. nov. is proposed. The type strain is NR4-ASC07(T) (= NBRC 112327(T) = TISTR 2465(T)).