Calidifontibacter terrae sp nov., an actinomycete isolated from soil, with potential applications in cosmetics

An aerobic, Gram-stain-positive, oxidase-and catalase-positive, non-motile, non-spore-forming, coccoid, creamish-whitecoloured bacterium, designated strain R161(T), was isolated from soil in Hwaseong, South Korea. The cell-wall peptidoglycan contained glycine, glutamic acid, alanine, aspartic acid, serine and lysine, and whole-cell sugars were galactose, rhamnose, glucose and ribose. Strain R161(T) showed antibacterial and enzyme inhibitory activities. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain R161(T) formed a lineage within the family Dermacoccaceae, and showed highest sequence similarity with type strains of Calidifontibacter indicus PC IW02(T) (97.71% sequence similarity) and Yimella lutea YIM 45900 T (97.58 %). The sequence similarity of strain R161 T with type strains of members of the genus Dermacoccus was less than 96.5 %. The major menaquinone was MK-8(H-4). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The major cellular fatty acids were iso-C16 : 0, anteiso-C-17 : 0, iso-C-16 : 1 H, anteiso-C-17 : 1 omega 9, summed feature 9 (iso-C-17 : 1 omega 9c and/or C-16 : 0 10-methyl) and iso-C-15 : 0. The DNA G+C content of strain R161(T) was 73.9 mol%. The DNA-DNA hybridization value between strain R161 T and C. indicus JCM 16038 T was 52.1 %. On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, strain R161 T represents a novel species of genus Calidifontibacter, for which the name Calidifontibacter terrae sp. nov. is proposed. The type strain of Calidifontibacter terrae sp. nov. is R161(T) (= KEMB 9005-404(T) = KACC 18906(T) = JCM 31558(T)).