Roseomonas nepalensis sp nov., isolated from oil-contaminated soil

A wine-red-coloured, Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain G-3-5(T), was isolated from oil-contaminated soil of Biratnagar, Morang, Nepal, during a study of oil-utilizing bacteria. This strain was catalase-negative and oxidase-positive. It was able to grow at 10-37 degrees C, at pH 6.0-10.0 and with 0.02-1.02% (w/v) NaCl. This strain was taxonomically characterized by a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain G-3-5(T) belongs to the genus Roseomonas and is closely related to Roseomonas vinacea CPCC 100056(T) (97.81% sequence similarity), Roseomonas aerilata 5420S-30(T) (96.68 %), Roseomonas pecuniae N75(T) (96.15 %), 'Roseomonas aceris' R-1 (95.75 %) and Roseomonas rosea 173/96(T) (95.30 %). The only respiratory quinone was ubiquinone-10. The polar lipid profile revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The major fatty acids of strain G-3-5(T) were summed feature 8 (C-18 : 1 omega 7c and/or C-18 : 1 omega 6c), summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c), C-16 : 0, C-18 : 0 and C-18 : 1 2-OH. The genomic DNA G+C content of this novel strain was 68.3 mol%. The DNA-DNA relatedness between strain G-3-5(T) and Roseomonas. vinacea KACC 13934(T) was 26.3 %. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain G-3-5(T) represents a novel species of the genus Roseomonas, for which the name Roseomonas nepalensis sp. nov. is proposed. The type strain is G-3-5(T) (= KEMB 9005-416(T) = KACC 18908(T) = JCM 31470(T)).