Journal
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Volume 67, Issue 5, Pages 1442-1450Publisher
MICROBIOLOGY SOC
DOI: 10.1099/ijsem.0.001838
Keywords
Actinomycetales; Antarctica; psychrotolerant; facultative anaerobe; ureolytic; chitinase
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Funding
- NASA
- National Science Foundation
- Arctic and Antarctic Research Institute (AARI) of the Russian Academy of Sciences
- Tawani Foundation International Schirmacher Oasis/Lake Antarctica Expedition
- Office of Science of the US Department of Energy [DE-AC02-05CH11231]
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A novel psychrotolerant bacterium, strain ISLP-3(T), was isolated from a sample of naturally formed ice sculpture on the shore of Lake Podprudnoye in Antarctica. Cells were motile, stained Gram-positive, non-spore-forming, straight or slightly curved rods with the shape of a baseball bat. The new isolate was facultatively anaerobic and catalase-positive. Growth occurred at 3-35 degrees C with an optimum at 22-24 degrees C, 0-2% (w/v) NaCl with an optimum at 0.3% and pH 6.2-9.5 with an optimum at pH 7.5. Strain ISLP-3(T) grew on several carbon sources, with the best growth on cellobiose. The isolate possessed ureolytic activity but growth was inhibited by urea. The strain was sensitive to: ampicillin, gentamycin, kanamycin rifampicin, tetracycline and chloramphenicol. Major fatty acids were: anteiso-C-15:0, iso-C-16:0, C-16:0, C-14:0 and iso-C-15:0. The predominant menaquinone was MK-9(H-4). The genomic G+C content was 69.5 mol%. The 16S rRNA gene showed 99% sequence similarity to that of Sanguibacter suarezii ST-26(T), but their recA genes shared <= 91% sequence similarity, suggesting that this new isolate represents a novel species within the genus Sanguibacter. This conclusion was supported by average nucleotide identity, which was <= 91% to the most closely related strain. The name Sanguibacter gelidistatuariae sp. nov. is proposed for the novel species with the type strain ISLP-3(T)=ATCC TSD-17(T) =DSM 100501(T) =JCM 30887(T)). The complete genome draft sequence of ISLP-3(T) was deposited under IMG OID 2657245272. Emendments to the descriptions of related taxa have been made based on experimental data from our comparative analysis.
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