Flavobacterium inkyongense sp nov., isolated from an artificial freshwater pond

A Gram-stain-negative, non-motile, yellow-pigmented bacterium, designated strain IMCC27201(T), was isolated from an artificial freshwater pond (Inkyong) in Korea. Growth of strain IMCC27201(T) occurred at 10-37 degrees C (optimum, 30 degrees C), at pH 7.0-8.0 (optimum, pH 7.0) and with 0-0.25% (w/v) NaCl (optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain IMCC27201(T) belonged to the genus Flavobacterium and was related most closely to Flavobacterium columnare IFO 15943(T) (97.8% sequence similarity), Flavobacterium terrae R2A1-13(T) (97.2 %) and Flavobacterium vireti THG-SM1(T) (96.4 %). Strain IMCC27201(T) exhibited low DNA-DNA relatedness with F. columnare KCTC 42885(T) (34.9-40.6 %). The DNA G+C content of strain IMCC27201(T) was 32.1 mol%. The major fatty acids were iso-C-15:0, iso-C-15 (: 1) G and iso-C-15 : 0 3-OH. Strain IMCC27201(T) contained menaquinone-6 (MK-6) as the sole isoprenoid quinone. The polar lipids of strain IMCC27201(T) consisted of phosphatidylethanolamine, three unidentified aminolipids and two unidentified lipids. Based on 16S rRNA gene phylogeny, phenotypic characterization and DNA-DNA relatedness, strain IMCC27201(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium inkyongense sp. nov. is proposed. The type strain is IMCC27201(T) (= KCTC 52244(T) = JCM 31385(T)).