Brevundimonas balnearis sp nov., isolated from the well water of a thermal bath

A novel alphaproteobacterium was isolated from the well water of a thermal bath at Budapest, Hungary. Phylogenetic analysis of the novel strain showed that this bacterium belongs to a distinct lineage among the genus Brevundimonas. Based on the 16S rRNA gene sequence strain FDRGB2b(T) showed the highest sequence similarity values to Brevundimonas naejangsanensis BIO-TAS2-2(T) (97.35 %), Brevundimonas viscosa F3(T) (97.28 %), Brevundimonas vesicularis LMG 2350(T) (97.27 %), Brevundimonas nasdae GTC 1043(T) (97.14 %), Brevundimonas vancanneytii LMG 2337(T) (97.13 %) and Brevundimonas aurantiaca DSM 4731(T) (97.13 %). The newly isolated bacterium was strictly aerobic, and its optimum growth occurred at 20-30 degrees C, between pH 8-9 and without NaCl. Movement was with a single polar flagellum, but the cells could also produce stalks. The major isoprenoid quinone of strain FDRGB2b(T) was Q-10, the major cellular fatty acids were C-18:1 omega 7c and C-16:0, and the polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol, two unknown phospholipids and four unknown glycolipids. The characteristic diamino acid in its cell wall is meso-diaminopimelic acid. The G+C content of DNA of the type strain was 69.8 mol%. Strain FDRGB2b(T) (=DSM 29841(T) = NCAIM B.02621(T)) is proposed as the type strain of a novel species with the proposed name Brevundimonas balnearis sp. nov.