Tenacibaculum haliotis sp nov., isolated from the gut of an abalone Haliotis discus hannai

A Gram-stain-negative, non-flagellated, gliding, non-spore-forming bacterial strain, designated RA3-2(T), was isolated from the gut of an abalone (Haliotis discus hannai) collected from the sea around Jeju island, South Korea, and subjected to a polyphasic taxonomic study. RA3-2(T) grew optimally at 20 degrees C and in the presence of 2.0-3.0% (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences indicated that RA3-2(T) fell within the clade comprising the type strains of species of the genus Tenacibaculum, clustering with the type strains of Tenacibaculum soleae, Tenacibaculum ovolyticum and Tenacibaculum dicentrarchi; showing 16S rRNA gene sequence similarity values of 96.2-96.8 %. The novel strain exhibited 16S rRNA gene sequence similarity values of 93.5-96.9% to the type strains of the other species of the genus Tenacibaculum. RA3-2(T) contained MK-6 as the predominant menaquinone and iso-C-15 : 0 and iso-C-15 : 0 3-OH as the major fatty acids. The major polar lipids of RA3-2(T) were phosphatidylethanolamine, two unidentified lipids, one unidentified aminophospholipid and one unidentified glycolipid. The DNA G+C content of RA3-2(T) was 31.7 mol%. The differential phenotypic properties, together with the phylogenetic data, revealed that RA3-2(T) is separated from other species of the genus Tenacibaculum with validly published names. On the basis of the data presented, RA3-2(T) is considered to represent a novel species of the genus Tenacibaculum, for which the name Tenacibaculum haliotis sp. nov. is proposed. The type strain is RA3-2(T) (= KCTC 52419(T) = NBRC 112382(T)).