Journal
STAR PROTOCOLS
Volume 1, Issue 1, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2019.100013
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Funding
- Research Scholar Grant from the American Cancer Society [RSG-14-171-01-CSM]
- NIH [U54CA210190, R01CA181385, R01CA181385S1]
- UMN College of Science and Engineering
- Masonic Cancer Center
- UMN Institute for Engineering in Medicine
- Randy Shaver Research and Community Fund
- NHLBI intramural research program
- National Science Foundation through the National Nano Coordinated Infrastructure Network (NNCI) [ECCS-1542202]
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We present a reproducible protocol for fabrication of polyacrylamide (PAA) hydrogel-based nano-patterns and nano-textures with a wide range of elastic rigidities to study fundamental cell behaviors, such as mechanosensitivity and motility. We explore the benefits of this protocol by successfully testing the compatibility of the PAA platforms with super-resolution microscopy, which is largely unavailable with platforms of nano-scale textures made from different polymers. We also utilized soft and rigid nano-textures to study the mechanosensing basis of T cell behavior and phenotype. For complete information on the generation and use of this protocol, please refer to Tabdanov et al. (2018b).
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