Journal
STAR PROTOCOLS
Volume 1, Issue 1, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2020.100022
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Funding
- Francis Crick Institute
- Francis Crick Institute from Cancer Research UK
- UK Medical Research Council [FC001198]
- Wellcome Trust [FC001198]
- ARC (Association de la Recherche Contre le Cancer)
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Amine-reactive Tandem Mass Tag 10plex (TMT10plex) labeling permits multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS/MS). We have used this technology to label 20 Saccharomyces cerevisiae samples collected in a time-resolved manner from a wild-type and phosphatase mutant background to characterize phosphoproteome dynamics. Here, we provide a detailed protocol for biological and mass spectrometry sample preparation and analysis. For complete details on the use and execution of this protocol, please refer to Touati et al. (2019).
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