Journal
STAR PROTOCOLS
Volume 1, Issue 1, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2020.100017
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Funding
- Lundbeck Foundation
- Novo Nordisk Foundation
- Danish National Research Foundation [DNRF107]
- European Union [787684]
- Marie Curie Actions (MSCA) [787684] Funding Source: Marie Curie Actions (MSCA)
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Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity. We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context. This protocol describes the use of the cell based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays. For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019).
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