Journal
NATURE PLANTS
Volume 6, Issue 7, Pages 766-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41477-020-0695-2
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Funding
- Academy of Finland [316544, 266431, 307335]
- European Research Council (ERC-CoG CORKtheCAMBIA) [819422]
- University of Helsinki HiLIFE fellowship
- European Molecular Biology Organisation (EMBO ALTF) [1046-2015]
- Chinese Scholarship Council
- European Research Council (ERC) [819422] Funding Source: European Research Council (ERC)
- Academy of Finland (AKA) [266431, 266431, 316544, 316544] Funding Source: Academy of Finland (AKA)
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Conditional manipulation of gene expression is a key approach to investigating the primary function of a gene in a biological process. While conditional and cell-type-specific overexpression systems exist for plants, there are currently no systems available to disable a gene completely and conditionally. Here, we present a new tool with which target genes can efficiently and conditionally be knocked out by genome editing at any developmental stage. Target genes can also be knocked out in a cell-type-specific manner. Our tool is easy to construct and will be particularly useful for studying genes having null alleles that are non-viable or show pleiotropic developmental defects.
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