Chemical Composition and Antimicrobial Activity Against Food Poisoning of Alcoholic Extract of Nigella Sativa L

Nowadays using natural sources for treating disease is one of the most favorable methods because of fewer side effects. Reporting antimicrobial properties of a new natural source can be useful for designing new natural medicines antimicrobial effect of Nigella Sativa L. seeds n-butanol extract will be useful for treating or preventing for many infections. In our study we report the first GC-MS analysis of n-butanol extract of Nigella Sativa L. seed and also its antimicrobial activity against some food poisoning and nosocomial infection causing microorganisms. Nigella Sativa L. seed powder was subjected to n-butanol with ratio 1:10 in a flask and then kept on shaker. After filtering, the extract has been analyzed by GC-MS. After that, the extract was tested against bacteria and fungi by three method.disc diffusion. well diffusion and micro dilution methods. The GC-MS analysis revealed that fatty acids and terpenoids are the major constituents. The result showed that psendomonos aureoginosa klebsiella, pneumoniae, acinetobacter baumanii. Yersinia enterocolitica, Candido albicans, Candido parapsilosis and Candida krusei were the sensitive microorganisms. According to great antimicrobial actin its and having nourishing components (fatty acids and terpenoids) adding this plant to a daily food diet is a good way for having a healthy life and this plant can be a new source for therapeutic uses.