Journal
FOOD & FUNCTION
Volume 11, Issue 6, Pages 5240-5256Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0fo00744g
Keywords
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Funding
- European Food Safety Authority [OC/EFSA/GMO/2017/01]
- School of Food Science and Nutrition at the University of Leeds
- Erasmus Plus Programme
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Evaluating the gastrointestinal (GI) fate of proteins is part of the assessment to determine whether proteins are safe to consume.In vitrodigestion tests are often used for screening purposes in the evaluation of potential allergenicity. However, the current pepsin resistant test used by the European Food Safety Authority, only corresponds to fasted gastric conditions representative of a late phase adult stomach. In addition, these tests are performed on isolated proteins and the effect of the food matrix and processing are not systematically considered. The aim of this research is to compare three different staticin vitroGI scenarios that are physiologically relevant. Namely, an infant, early phase (fed state) adult and late phase (fasted state) adult model. These protocols are applied to well-characterised isolated dairy (beta-lactoglobulin and beta-casein) and egg (lysozyme and ovalbumin) proteins and the impact of food matrix/processing on their proteolysis is also investigated. A combination of SDS-PAGE, LC-MS/MS and spectrophotometric assay was used for the evaluation of the proteolysis. Results highlight differences across the three GI scenarios whether on isolated proteins or within food matrices. The infant model led to incomplete digestion, leaving intact egg proteins, either isolated or in the food matrix, and intact beta-lactoglobulin in the milk. In addition, peptides greater than 9 amino acids were found throughout the intestinal phase for all proteins studied, regardless of the scenario. This reinforces the difficulty of linking protein digestibility to potential allergenicity because many other factors are involved that need further investigation.
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