Journal
INTERNATIONAL JOURNAL OF NEUROSCIENCE
Volume 128, Issue 2, Pages 125-132Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/00207454.2017.1375913
Keywords
Annexin A10; ERK1/2; TNF-alpha; IL-1 beta; pain
Categories
Funding
- Medical and Health Technology Project of Yancheng [YK2015029]
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Purpose: The current study aims at investigating the downstream targets of spinal Annexin A10 in modulating neuropathic pain. Materials and methods: Paw withdrawal latency and paw withdrawal threshold were measured to evaluate the pain-associated behaviour in rats. The expression of spinal Annexin A10, phosphorylated-extracellular regulated kinase 1/2 and extracellular regulated kinase were detected by western blotting. The level of tumour necrosis factor-a and interleukine-1 beta was tested by enzyme-linked immunosorbent assay (ELISA) kits. Results: Chronic constrictive injury caused pain hypersensitivity in rats, along with increased expression of spinal Annexin A10, phosphorylated-extracellular regulated kinase 1/2, tumour necrosis factor-alpha and interleukine-1 beta in rats. Knockdown of spinal Annexin A10 suppressed the chronic constrictive injury-induced hyperalgesia, and inhibited the chronic constrictive injuryinduced increased expression of phosphorylated-extracellular regulated kinase 1/2, tumour necrosis factor-alpha and interleukine-1 beta in the spinal cord. Inhibition of spinal extracellular regulated kinase activation decreased the release of tumour necrosis factor-a and interleukine-1 beta, but did not change the increased expression of Annexin A10 caused by chronic constrictive injury. Conclusions: Annexin A10 contributed to the development of neuropathic pain by activating spinal extracellular regulated kinase signalling and the subsequent release of tumour necrosis factor-alpha and interleukine-1 beta in the spinal cord.
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