Journal
INTERNATIONAL JOURNAL OF NANOMEDICINE
Volume 12, Issue -, Pages 795-808Publisher
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S124938
Keywords
protein desolvation; nanoparticle assembly; gelatin nanoparticle synthesis; protease susceptibility; intracellular drug release
Funding
- Department of Science and Technology (DST), Government of India
- Council for Scientific and Industrial Research (CSIR)
- Sir JC Bose National Fellowship of the Department of Science and Technology (DST), India
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The process of moving hydrophobic amino acids into the core of a protein by desolvation is important in protein folding. However, a rapid and forced desolvation can lead to precipitation of proteins. Desolvation of proteins under controlled conditions generates nanoparticles - homogeneous aggregates with a narrow size distribution. The protein nanoparticles, under physiological conditions, undergo surface erosion due to the action of proteases, releasing the entrapped drug/gene. The packing density of protein nanoparticles significantly influences the release kinetics. We have investigated the desolvation process of gelatin, exploring the role of pH and desolvating agent in nanoparticle synthesis. Our results show that the desolvation process, initiated by the addition of acetone, follows distinct pathways for gelatin incubated at different pH values and results in the generation of nanoparticles with varying matrix densities. The nanoparticles synthesized with varying matrix densities show variations in drug loading and protease-dependent extra-and intracellular drug release. These results will be useful in fine-tuning the synthesis of nanoparticles with desirable drug release profiles.
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