Production of formate from CO2gas under ambient conditions: towards flow-through enzyme reactors

A flow-through bi-enzymatic reactor was developed to produce formate from carbon dioxide gas (CO2). The reaction operates under ambient conditions without hydrogen gas (H-2) and involves a high performance formate dehydrogenase fromMethylobacterium extorquensAM1 (MeFoDH1). The tandem reaction featuresin situregeneration of the nicotinamide adenine dinucleotide (1,4-NADH) cofactor performed by a highly active mutant of the phosphite dehydrogenase fromPseudomonas stutzeri, without any side reaction. One of the highest turnover numbers reported to date in the literature was reached (6700) giving a high space-time yield of 0.6 mmol(formate)min(-1)L(reactor)(-1).