Journal
RSC ADVANCES
Volume 10, Issue 40, Pages 23931-23935Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0ra02382e
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Funding
- Romanian National Authority for Scientific Research and Innovation, CNCS UEFISCDI [PN-II-RU-TE-2014-4-0808]
- University of Bucharest
- EEA Grants 2014-2021, the SmartVac Project [1/2019]
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We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC). The resin was found to be highly efficient for purification of His-tagged HCV E2 glycoproteins expressed in 293T mammalian cells.
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