Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 18, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/ijms18071473
Keywords
fluorogenic probes; protein labeling; RNA labeling
Funding
- European Research Council [ERC-2016-CoG-724705 FLUOSWITCH]
- Agence National de la Recherch [ANR-14-CE09-0002-01]
- PSL Research University [IMRESOV]
- France BioImaging
- Region Ile-de-France
- Equipex Morphoscope 2 [ANR-11-EQPX-0029]
- Agence Nationale de la Recherche (ANR) [ANR-14-CE09-0002] Funding Source: Agence Nationale de la Recherche (ANR)
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The spatiotemporal fluorescence imaging of biological processes requires effective tools to label intracellular biomolecules in living systems. This review presents a brief overview of recent labeling strategies that permits one to make protein and RNA strongly fluorescent using synthetic fluorogenic probes. Genetically encoded tags selectively binding the exogenously applied molecules ensure high labeling selectivity, while high imaging contrast is achieved using fluorogenic chromophores that are fluorescent only when bound to their cognate tag, and are otherwise dark. Beyond avoiding the need for removal of unbound synthetic dyes, these approaches allow the development of sophisticated imaging assays, and open exciting prospects for advanced imaging, particularly for multiplexed imaging and super-resolution microscopy.
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