Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 18, Issue 1, Pages -Publisher
MDPI AG
DOI: 10.3390/ijms18010226
Keywords
lncRNA; RHPN1-AS1; uveal melanoma; migration
Funding
- National Natural Science Foundation of China [81500757, 81502481, 81372909, 81570884, 31470757, 81372469, U1432117, 81602366]
- Scientific Research Program of National Health and Family Planning Commission of China [2014040]
- Program for Professor of Special Appointment (Eastern Scholar) at the Shanghai Institutions of Higher Learning [1410000159]
- Dawn Program of Shanghai Education Commission [14SG18]
- SMC-ChenXing Yong Scholar Program
- Shanghai Municipal Education Commission-Gaofeng Clinical Medicine [20161317]
- Science and Technology Commission of Shanghai [14JC1404100, 14JC1404200, 14430723100]
- Outstanding Yong Scholar Grant of Shanghai Jiao Tong University School of Medicine [16XJ11002]
- Foundation of Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine [2014A05]
- Shanghai Jiao Tong University School of Medicine Doctor Innovation Fund [BXJ201432]
Ask authors/readers for more resources
Increasing evidence suggests that aberrant long non-coding RNAs (lncRNAs) are significantly correlated with the pathogenesis, development and metastasis of cancers. RHPN1 antisense RNA 1 (RHPN1-AS1) is a 2030-bp transcript originating from human chromosome 8q24. However, the role of RHPN1-AS1 in uveal melanoma (UM) remains to be clarified. In this study, we aimed to elucidate the molecular function of RHPN1-AS1 in UM. The RNA levels of RHPN1-AS1 in UM cell lines were examined using the quantitative real-time polymerase chain reaction (qRT-PCR). Short interfering RNAs (siRNAs) were designed to quench RHPN1-AS1 expression, and UM cells stably expressing short hairpin (sh) RHPN1-AS1 were established. Next, the cell proliferation and migration abilities were determined using a colony formation assay and a transwell migration/invasion assay. A tumor xenograft model in nude mice was established to confirm the function of RHPN1-AS1 in vivo. RHPN1-AS1 was significantly upregulated in a number of UM cell lines compared with the normal human retinal pigment epithelium (RPE) cell line. RHPN1-AS1 knockdown significantly inhibited UM cell proliferation and migration in vitro and in vivo. Our data suggest that RHPN1-AS1 could be an oncoRNA in UM, which may serve as a candidate prognostic biomarker and target for new therapies in malignant UM.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available