Journal
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Volume 40, Issue 2, Pages 411-417Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/ijmm.2017.3027
Keywords
heme oxygenase-1; hemin; transforming growth factor-beta; epithelial-mesenchymal transition; breast cancer
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Funding
- National Natural Science Foundation of China [81172337]
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Epithelial-mesenchymal transition (EMT) is a key mechanism underlying metastatic breast cancer. Reactive oxygen species (ROS) play an important role in EMT. Heme oxygenase-1 (HMOX-1) can reduce oxidative stress. However, the effect of HMOX-1 on the EMT process in breast cancer cells is unknown. We treated the MCF-7 breast cancer cell line with the HMOX-1 inducer hemin and observed that hemin induced HMOX-1 expression and inhibited migration, invasion and ROS generation in transforming growth factor-beta (TGF-beta)-treated MCF-7 cells using quantitative RT-qPCR, western blotting, wound-healing and cell invasion assays as well as fluorescent probe DCFDA. Hemin inhibited TGF-beta-induced EMT in the MCF-7 cells, whereas HMOX-1 siRNA attenuated the suppressive effect of hemin as determined by the expression and cellular distribution of selected EMT markers. In summary, our results revealed that hemin treatment increased HMOX-1 expression and inhibited TGF-beta-induced EMT in MCF-7 cells.
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