Journal
ARCHIVES OF BIOLOGICAL SCIENCES
Volume 72, Issue 2, Pages 211-221Publisher
INST BIOLOSKA ISTRAZIVANJA SINISA STANKOVIC
DOI: 10.2298/ABS191216015W
Keywords
Eucommia ulmoides leaves; flavonoids; ultrasound-microwave-assisted extraction; antioxidant activity; antibacterial activity
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Funding
- National and Local United Engineering Laboratory of Integrative Utilization Technology of E. ulmoides [NLE201602, NLE201704]
- National Natural Science Foundation of China [31660181]
- Key Laboratory of Human Forest Products and Chemical Industry Engineering [LCHG1902]
- Educational Commission of Hunan Province of China [19C1532]
- GDAS Project of Science and Technology Development [2019GDASYL-0502003]
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Ultrasound/microwave assisted extraction (UMAE) of flavonoid compounds from Eucommia ulmoides leaves was studied and the extraction conditions were optimized by the Plackett-Burman design (PBD) method combined with the Box-Behnken design (BBD). The antioxidant and antibacterial activities of the flavonoid extract were investigated. The results show that the optimal conditions were an ethanol concentration of 41%, microwave power of 178 W and an ultrasound extraction time of 26 min. Under these conditions, the yield of the flavonoid compounds was 2.454%+/- 0.230%, which was higher than that after direct solvent extraction, ultrasound extraction and microwave extraction. The results of in vitro antioxidant assays showed that the flavonoid extract had scavenging capacity for DPPH, ABTS and hydroxyl radicals, with corresponding IC50 values of 30.76 mg/L, 21.09 mg/L, 248.4 mg/L, respectively. In addition, this extract exhibited strong antibacterial activity on Escherichia coli, Staphylococcus aureus and Salmonella typhimurium.
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