Journal
CHEMICAL COMMUNICATIONS
Volume 56, Issue 54, Pages 7515-7518Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0cc01432j
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Funding
- National Natural Science Foundation of China [91853111, 21922701, 21778005]
- National Major Scientific and Technological Special Project [2019ZX09739001]
- Clinical Medicine Plus X -Young Scholars Project of Peking University [PKU2020LCXQ029]
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The CRISPR-Cas9 system enables facile and efficient genome engineering in living cells and organisms. We report a Cas9-RNA conjugation strategy to afford minimal crRNA containing only the guide sequence for the target gene, which may simplify and reduce the cost for large-scale and high-throughput crRNA synthesis and lead to new insights into the design of CRISPR family complexes for diverse purposes.
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