Journal
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
Volume 56, Issue -, Pages 274-282Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ijid.2017.01.024
Keywords
Mycobacterium tuberculosis; tuberculosis; vaccines; Bacille Calmette-Guerin; antigens; T cells; antibodies
Categories
Funding
- Swedish Heart and Lung Foundation (Hjartlungfonden)
- Vinnova
- Swedish Research Council (Vetenskapradet)
Ask authors/readers for more resources
Objectives: Advances in tuberculosis (TB) vaccine development are urgently required to enhance global disease management. We evaluated the potential of Mycobacterium tuberculosis (M. tb)-derived protein antigens Rv0447c, Rv2957 and Rv2958c to boost BCG vaccine efficacy in the presence or absence of glucopyranosyl lipid adjuvant formulated in a stable emulsion (GLA-SE) adjuvant. Methods: Mice received the BCG vaccine, followed by Rv0447c, Rv2957 and Rv2958c protein boosting with or without GLA-SE adjuvant 3 and 6 weeks later. Immune responses were examined at given time points. 9 weeks post vaccination, mice were aerosol-challenged with M. tb, and sacrificed at 6 and 12 weeks to assess bacterial burden. Results: Vaccination of mice with BCG and M. tb proteins in the presence of GLA-SE adjuvant triggered strong IFN-gamma and IL-2 production by splenocytes; more TNF-alpha a was produced without GLA-SE addition. Antibody responses to all three antigens did not differ, with or without GLA-SE adjuvant. Protein boosting without GLA-SE adjuvant resulted in vaccinated animals having better control of pulmonary M. tb load at 6 and 12 weeks post aerosol infection, while animals receiving the protein boost with GLA-SE adjuvant exhibited more bacteria in the lungs. Conclusions: Our data provides evidence for developing Rv2958c, Rv2957 and Rv0447c in a heterologous prime-boost vaccination strategy with BCG. (C) 2017 Published by Elsevier Ltd
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available