Journal
NANOSCALE
Volume 12, Issue 16, Pages 8647-8655Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9nr08060k
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Funding
- University of Leeds
- Wellcome Trust [097354/Z/11/Z]
- Biotechnology and Biological Sciences Research Council [BB/R007829/1]
- EU [797597]
- Wellcome Trust [097354/Z/11/Z] Funding Source: Wellcome Trust
- Marie Curie Actions (MSCA) [797597] Funding Source: Marie Curie Actions (MSCA)
- BBSRC [BB/R007829/1] Funding Source: UKRI
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Full water-dispersion of commercial hydrophobic CdSe/CdS core/shell quantum rods (QRs) was achieved by cap-exchange using a dihydrolipoic acid zwitterion ligand at a low ligand:QR molar ratio (LQMR) of 1000. However, this process almost completely quenched the QR fluorescence, greatly limiting its potential in downstream fluorescence based applications. Fortunately, we found that the QR fluorescence could be recovered by exposure to near ultra-violet to blue light radiation (e.g. 300-450 nm). These reborn QRs were found to be compact, bright, and stable, and were resistant to nonspecific adsorption, which make them powerful fluorescent probes in broad biomedical applications. We demonstrated their potential in two model applications: first, the QRs were conjugated with His(8)-tagged small antibody mimetic proteins (also known as Affimers) for the sensitive detection of target proteins via a Forster resonance energy transfer (FRET) readout strategy and second, the QR surface was functionalized with biotins for targeted imaging of cancer cells.
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