Journal
JOURNAL OF IMMUNOTOXICOLOGY
Volume 17, Issue 1, Pages 153-162Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/1547691X.2020.1776428
Keywords
Hog barn dust; swine; lung; macrophage; TNF-alpha; IL-10; CD204; PKC zeta
Categories
Funding
- NIOSH, Central States Center for Agricultural Safety and Health [CS-CASH] [U54 OH010162]
- NIEHS [R01 ES019325]
- Department of Veterans Affairs (VA) Merit Award [I01 BX003635, I01 CX001714]
- Research Career Scientist Award from the Department of Veterans Affairs [IK6 BX003781]
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Exposure to organic dust increases chronic airway inflammatory disorders. Effective treatment strategies are lacking. It has been reported that hog barn dust extracts (HDE) induce TNF alpha through protein kinase C (PKC) activation and that lung inflammation is enhanced in scavenger receptor A (SRA/CD204) knockout (KO) mice following HDE. Because interleukin (IL)-10 production can limit excessive inflammation, it was hypothesized here that HDE-induced IL-10 would require CD204 to effect inflammatory responses. C57BL/6 wild-type (WT), SRA KO, and IL-10 KO mice were intranasally challenged daily for 8 days with HDE and subsequently rested for 3 days with/without recombinant IL-10 (rIL-10) treatment. Primary peritoneal macrophages (PM) and murine alveolar macrophages (MH-S cells) were treatedin vitrowith HDE, SRA ligand (fucoidan), rIL-10, and/or PKC isoform inhibitors. HDE inducedin vivolung IL-10 in WT, but not SRA KO mice, and similar trends were demonstrated in isolated PM from same treated mice. Lung lymphocyte aggregates and neutrophils were elevated inin vivoHDE-treated SRA and IL-10 KO mice after a 3-d recovery, and treatment during recovery with rIL-10 abrogated these responses.In vitrorIL-10 treatment reduced HDE-stimulated TNF alpha release in MH-S and WT PM. In SRA KO macrophages, there was reduced IL-10 and PKC zeta (zeta) activity and increased TNF alpha followingin vitroHDE stimulation. Similarly, blocking SRA (24 hr fucoidan pre-treatment) resulted in enhanced HDE-stimulated macrophage TNF alpha and decreased IL-10 and PKC zeta activation. PKC zeta inhibitors blocked HDE-stimulated IL-10, but not TNF alpha. Collectively, HDE stimulates IL-10 by an SRA- and PKC zeta-dependent mechanism to regulate TNF alpha. Enhancing resolution of dust-mediated lung inflammation through targeting IL-10 and/or SRA may represent new approaches to therapeutic interventions.
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