Journal
BIONANOSCIENCE
Volume 10, Issue 3, Pages 683-689Publisher
SPRINGER
DOI: 10.1007/s12668-020-00753-x
Keywords
Green nanoparticles; Apoptosis; Cytotoxicity; Acacia luciana; Bak; Bcl ratio
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Due to unique properties of green silver nanoparticles, especially chemical and biological properties, they are one of the most used metallic nanoparticles in modern healthcare applications. Accordingly, we synthesized Ag-NPs usingAcacia lucianaas a reducing and stabilizing agent, to elucidate its apoptosis inducing ability in MCF-7 cancer cells. The Ag-NPs were characterized using FTIR and FESEM. These instruments confirmed that biosynthesized nanoparticles are rod-shaped with average size of 50 nm and negative surface charge of 2.59 mV. The in vitro anticancer effects using MTT assay revealed that the Ag-NPs showed inhibition of cell survival in a time and dose-dependent manner with IC(50)value of 4.37 mg/mL. Also, the expression of pro- and antiapoptotic genes was measured using real-time PCR. The results hinted that Ag-NPs can significantly increase the Bak1/Bclx ratios through the change in mitochondrial membrane permeability and be used as a therapeutic agent breast cancer cells.
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