Journal
CURRENT OPINION IN CHEMICAL BIOLOGY
Volume 25, Issue -, Pages 55-64Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2014.12.036
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Funding
- NSF under the CCI Center for Enabling New Technologies through Catalysis (CENTC) [CHE-0650456, CHE-1205189]
- Dow Chemical
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1205189] Funding Source: National Science Foundation
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Over the last two decades, directed evolution has become a staple in protein engineering and ushered in a new era of industrial biocatalysis. Directed evolution has provided the tools to not only improve the activity of known biocatalysts, but also to endow biocatalysts with chemical reactivities not previously encountered in nature. Here we will discuss the recent successes in the quest to enhance thermostability, stereoselectivity and activity of biocatalysts, as well as to create novel enzymes, over the last two years.
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