Journal
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
Volume 261, Issue -, Pages 42-48Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijfoodmicro.2017.09.002
Keywords
ZnFe2O4-reduced graphene oxide nanostructures; Aptamer; Salmonella enterica serovar typhimurium; Peroxidase mimetics; Colorimetric
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Funding
- Natural Science Foundation of Jiangsu Province [BK20140155]
- Key Research and Development Program of Jiangsu Province [BE2016306]
- Jiangsu Planned Projects for Postdoctoral Research Funds [1601087B]
- China Postdoctoral Science Foundation [2017M610299, 2016 T90430, 2015M580402]
- National Natural Science Foundation of China (NSFC) [31401575]
- Collaborative innovation center of food safety and quality control in Jiangsu Province
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A new colorimetric aptasensor platform was fabricated to detect Salmonella enterica serovar typhimurium based on the peroxidase-like activity of ZnFe2O4-reduced graphene oxide (ZnFe2O4/rGO) nanostructures. The synthesized ZnFe2O4/rGO can catalytically oxidize 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2 and generate a typical blue product, which was detected by micro-reader at 652 nm. Therefore, ZnFe2O4/rGO was conjugated with aptamer to act as the signal probe. Biotin modified aptamer was immobilized onto the micro-plate to act as the capture probe. In the presence of target, a sandwich-type complex of aptamer (on micro-plate)-target-aptamerZnFe(2)O(4)/rGO complexes was formed through specific recognition of aptamers and corresponding target. The limit of detection was 11 cfu/mL and the detection range was 11 to 1.10 x 10(5) cfu/mL of S. typhimurium in buffer solution. Actual samples analysis indicated that this colorimetric aptasensor produced results consistent with plate-counting analysis. The developed method was simple and sensitive, which may pave the way for the detection of other pathogenic bacteria with suitable aptamers.
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