Journal
CURRENT OPINION IN BIOTECHNOLOGY
Volume 32, Issue -, Pages 76-84Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2014.11.007
Keywords
-
Funding
- Gatsby Charitable Foundation
- European Research Council (ERC)
- Biotechnology and Biological Sciences Research Council (BBSRC)
- BBSRC [BBS/E/T/000PR6193, BB/L014130/1, BBS/E/T/000PR5885] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/T/000PR5885, BBS/E/T/000PR6193] Funding Source: researchfish
Ask authors/readers for more resources
CRISPR/Cas9 is a rapidly developing genome editing technology that has been successfully applied in many organisms, including model and crop plants. Cas9, an RNA-guided DNA endonuclease, can be targeted to specific genomic sequences by engineering a separately encoded guide RNA with which it forms a complex. As only a short RNA sequence must be synthesized to confer recognition of a new target, CRISPR/Cas9 is a relatively cheap and easy to implement technology that has proven to be extremely versatile. Remarkably, in some plant species, homozygous knockout mutants can be produced in a single generation. Together with other sequence-specific nucleases, CRISPR/Cas9 is a game-changing technology that is poised to revolutionise basic research and plant breeding.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available