Journal
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
Volume 12, Issue 7, Pages 6245-6254Publisher
ESG
DOI: 10.20964/2017.07.69
Keywords
Electrochemical behaviors; heme; detection; hydrogen bond
Categories
Funding
- National Natural Science Foundation of China [61661014, 61301038, 61271119]
- National Major Scientific Research Instruments Development Program [61627807]
- Natural Science Foundation of Guangxi Province [2015GXNSFBA139041, 2013GXNSFAA019224]
- National Undergraduate Training Programs for Innovation and Entrepreneurship [201510595044, 201510595207]
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Heme is an active center of hemoglobin which is a porphyrin compound containing two free carboxyl groups that may exert intermolecular interaction with ferrocene dicarboxylate which can be studied by electrochemical methods. The CV experimental results from this current study showed that ferrocene dicarboxylate had a good pair of redox peaks in NaOH aqueous solution and organic solvents in a quasi-reversible electrode process. There was linear relationship between current and square root of the scan rate, belonging to diffusion-controlled redox processes. The signals from the potential and current had obvious shift after the reaction with heme, especially when the redox peaks disappeared from the organic solvents. Fc(COOH)(2) was activated to give compound Fc(OBt)(2) with saturated structure that did not show better redox peaks due to its worse solubility, hence it didn't form hydrogen bonds with heme. DPV was used for heme assay, where the current was proportional to heme concentration in the range of 1.0-5.0 mu M, with 0.7 nM detection limits.
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