Journal
CHEMICAL COMMUNICATIONS
Volume 56, Issue 61, Pages 8631-8634Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0cc02181d
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- Ministry of Culture and Science within the framework of the NRW Strategieprojekt BioSC [313/323-400-00213]
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A colourimetric high-throughput screening system was established for directed evolution of prodigiosin ligase PigC. The two-step system consists of a colony prescreening test and a subsequent photometric 96-well plate assay. Screening PigC epPCR libraries inPseudomonas putidarevealed a PigC variant that achieved a 2.9x increased yield of prodiginine derivatives.
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