Journal
RSC ADVANCES
Volume 10, Issue 47, Pages 28351-28354Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0ra05406b
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Funding
- German Research Foundation (DFG) within the priority program SPP 1708 Material Synthesis Near Room Temperature [HE 7165/7-1, JA 466/31-2]
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We describe a holistic approach for achieving a nearly quantitative conversion for an enzymatic reaction while simultaneously increasing the long-term stability of the enzyme. The approach provided chemical control of bioreactions by utilizing newly synthesized tetrahydrothiophene-based ionic liquids (THT ILs). We showcased its power by using THT-ILs as additives at a low concentration (only 10 mmol L-1) in the alcohol dehydrogenase (ADH)-catalyzed synthesis of methylated 1-phenylethanol (Me-PE). We discovered an odd-even effect of the IL-cation chain length: Me-PE displayed beneficial interactions with THT ILs having odd-numbered chain lengths and deleterious interactions with those having even-numbered chain lengths. An intermolecular thermodynamic simulation of the bulk phase and critical micelle concentration investigations of the local surroundings of the THT-ILs proved the occurrence of these interactions, and these two methods confirmed the odd-even effect from different perspectives. Additionally, storing the ADH enzyme in pure THT IL at room temperature allowed for a boosted long-term stability of the enzyme (500 times greater than that in aqueous buffer) without the need for freezing.
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