Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
Volume 13, Issue 12, Pages 1470-1478Publisher
IVYSPRING INT PUBL
DOI: 10.7150/ijbs.21312
Keywords
genome editing; user-defined protospacer adjacent motif; off-target
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Funding
- National High Technology Research and Development Program of China [863 Program] [2013AA102502]
- National Transgenic Project of China [2016ZX08006003-004]
- National Key Research and Development Program of China, Stem Cell and Translational Research [2016YFA0100203]
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Designing efficient and specific CRISPR single-guide RNAs (sgRNAs) is vital for the successful application of CRISPR technology. Currently, a growing number of new RNA-guided endonucleases with a different protospacer adjacent motif (PAM) have been discovered, suggesting the necessity to develop a versatile tool for designing sgRNA to meet the requirement of different RNA-guided DNA endonucleases. Here, we report the development of a flexible sgRNA design program named CRISPR-offinder. Support for user-defined PAM and sgRNA length was provided to increase the targeting range and specificity. Additionally, evaluation of on-and off-target scoring algorithms was integrated into the CRISPR-offinder. The CRISPR-offinder has provided the bench biologist a rapid and efficient tool for identification of high quality target sites, and it is freely available at https://sourceforge.net/projects/crispr-offinder-v1-2/ or http://www.biootools.com.
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