Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 104, Issue -, Pages 1813-1819Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2017.06.043
Keywords
Chitosan nanoparticles; PONNEEM; Botanical pesticide
Funding
- Times of India-Loyola College, Chennai, India project
- ISPP, King Saud University, Saudi Arabia (ISPP) [0020]
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A nanopesticide formulation was developed using chitosan and a botanical pesticide PONNEEM (R) and its antifeedant, larvicidal and growth regulating activities were screened against Helicoverpa armigera, a major lepidopteran pest. Chitosan nanoparticles (CSNs) were prepared by using two different cross linking agents namely glutaraldehyde (GLA) and tripolyphosphate (TPP). The effects of cross linking agents on CSNs and the biological properties against the insect pest were also studied. Cross linking of chitosan with either TPP or GLA was confirmed through Fourier Transform Infrared (FTIR) spectroscopy. Electron micrograph revealed that the size of CSNs varied from 32 to 90 nm. The stability of nanopartides lasted for 9 days in CSNs-TPP-PONNEEM. In CSNs-GLA-PONNEEM, the stability of nanoparticles was higher. CSNs-TPP-PONNEEM treatment recorded 88.5% antifeedant activity and 90.2% larvicidal activity against H. armigera. Weights of H. armigera pupae in CSNs-TPP-PONNEEM treatment were significantly low. Chitosan-based nano-pesticide formulation holds great promise in H. armigera management. (C) 2017 Elsevier B.V. All rights reserved.
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