Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 104, Issue -, Pages 1238-1245Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2017.06.118
Keywords
F. solani; A. fumigatus; Cutinase; Characterization; Biodegradation; Biosynthesis
Funding
- China Spark Program [2015GA710001]
- National Natural Science Foundation of China [21007061]
- opening foundation of Key Laboratory of Recycling and Eco-treatment of Waste Biomass of Zhejiang Province [2016REWB08]
- Natural Science Foundation of Zhejiang Province [2016REWB08]
- Program for International S&T Cooperation Projects of China [2014DFE90040]
- Key Project in the Youth Elite Support Plan of the Zhenjiang Academy of Agricultural Sciences
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In this study, two synthesized cutinase genes from Fusarium solani and Aspergillus fumigatus were expressed in Pichia pastoris X33. The characteristics of these two cutinases were investigated and compared. The results indicated that F. solani and A. fumigatus cutinases hydrolyzed p-nitrophenyl substrates with different carbon chain lengths. A. fumigatus cutinase predominately hydrolyzed p-nitrophenyl butyrate, but F. solani cutinase preferred p-nitrophenyl decanoate. The abilities of polymer synthesis and bioplastic degradation were tested and compared between F. solani and A. fumigatus cutinases. The results showed that F. solani cutinase had degradation ability on poly(epsilon-caprolactone) (PCL) and synthesized polymer with a molecular weight (MW) of 2300 in organic solvent. However, A. fumigatus cutinase completely degraded PCL and synthesized molecules with a MW of 25,000, suggesting that A. fumigatus cutinase has more promising applications. (C) 2017 Elsevier B.V. All rights reserved.
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