Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 94, Issue -, Pages 1-9Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2016.09.099
Keywords
Polysaccharide; Ganoderma lucidum; Intestinal barrier function
Funding
- Fundamental Research Funds for the Central Universities [3102014JCQ15001, 3102015BJ(II)MYZ29]
- China Postdoctoral Science Foundation [2011M501481, 2012T50821]
- Shaanxi Provincial Natural Science Foundation [2015JQ3083]
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The intestinal mucosal barriers play essential roles not only in the digestion and absorption of nutrients, but also the innate defense against most intestinal pathogens. In the present study, polysaccharide from the mycelia of Ganoderma lucidum was given via oral administration to rats (100 mg/kg body weight, 21 days) to investigate its effects on intestinal barrier functions, including the mechanical barrier, immunological barrier and biological barrier function. It was found that the polysaccharide administration could significantly up-regulate the expression of occludin, nuclear factor-kappa B p65 (NF-kappa B p65) and secretory immunoglobulin A (SIgA) in ileum, markedly improve the levels of interferon-gamma (IFN-gamma), interleukin-2 (IL-2), and IL-4, and decrease the level of diamine oxidase (DAO) in serum. Meanwhile, rats from the polysaccharide group showed significant higher microbiota richness in cecum as reflected by the Chao 1 index compared with the control group. Moreover, the polysaccharide decreased the Firmicutesto-Bacteroidetes ratio. Our results indicated that the polysaccharide from the mycelia of G. lucidum might be used as functional agent to regulate the intestinal barrier functions. (C) 2016 Elsevier B.V. All rights reserved.
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