Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 102, Issue -, Pages 847-856Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2017.04.031
Keywords
Dioscorea hemsleyi polysaccharides; Extraction techniques; Antioxidant and antidiabetic activity
Funding
- National Natural Science Foundation of China [81173487, 81373904]
- Science and Technology Program of China [2014FY111100]
- Special Scientific Research of Chinese Medicine Industry [201307008]
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The rhizoma of Dioscorea hemsleyi (DH) has been used as a treatment of diabetes in China for hundreds of years. Polysaccharides in DH were extracted by using ultrasonic-assisted extraction (UAE), cold water extraction (CWE), warm water extraction (WWE) and hot water extraction (HWE), separately. Then the different characterizations of four DH polysaccharide (DHP) samples were analyzed by high-performance liquid chromatography (HPLC), high-performance Gel permeation chromatography (HGPC), ultraviolet-visible spectroscopy(UV), fourier transform-infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). Their activities in vitro of DHP were compared. Experimental results showed that HWE had the highest yield and large molecular weight. OWE had the highest uronic acid yield and little molecular weight, and its DPPH, AGI and MI activity were the best. The molecular weight of UAE was small, and its RP and FRAP activity were the best. Four DHP samples had differences in the surface topography, while they all had the typical IR spectra characteristic of polysaccharides. According the correlation analysis, it showed that the more uronic acid and the lower molecular weight was, the higher the antioxidant activity was. The high content of monosaccharide composition of Xyl, Ara, GIcA and GalA, and little molecular weight have good effect on antidiabetic activity. (C) 2017 Elsevier B.V. All rights reserved.
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