Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 104, Issue -, Pages 377-383Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2017.06.053
Keywords
Trametes versicolor polysaccharide; Farnesol; Biological activities
Funding
- National Basic Research Program (973 Program) of China [2013CB733600]
- National Natural Science Foundation of China [21476242]
- National Key Technology Research and Development Program of China [2015BAK45B01]
- National High Technology Research and Development Program (863 Program) of China [2012AA101803]
- Chinese Academy of Sciences [KSZD-EW-Z-015]
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A novel strategy of exposing 2-day-old mycelia cultures to 0.8 mM farnesol was developed to stimulate extracellular polysaccharide (EPS) production in Trametes versicolor submerged cultures. Farnesol, a quorum sensing molecule in fungi, could significantly increase EPS production by promoting polysaccharide biosynthesis and regulating mycelial morphology. EPS yield reached a maximum of 2.56 g/L that was 2.7-fold greater than that of control cultures. Farnesol made T. versicolor develop into fluffy, loose and multi-hyphae morphology, which facilitated the excretion of intracellular polysaccharide into culture medium. Moreover, EPS from farnesol-induced cultures (EPS-F) with higher carbohydrate and uronic acid contents mainly contained high molecular weight polysaccharide (134 kDa, 85%), and comprised glucose, mannose and galactose in a molar ratio of 34.2:2.1:1.0. These physicochemical properties led to stronger antioxidant and antitumor activities of EPS-F. This is the first report that farnesol can significantly improve the production of polysaccharide with higher biological activities. It provides a novel strategy to enhance the production and bioactivity of mushroom polysaccharide using microbial quorum sensing molecules. (C) 2017 Elsevier B.V. All rights reserved.
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